Immune receptors : methods and protocols /
Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity. The receptors that mediate these functions are at the front lines of both protective and regulative roles of the immune system. In...
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| Other Authors: | , , , |
| Format: | Book |
| Language: | English |
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New York :
Humana : Springer,
©2011
Totowa, NJ : Humana Press : Imprint: Humana Press, 2011 |
| Series: | Methods in Molecular Biology, Methods and Protocols,
748 Methods in molecular biology (Clifton, N.J.) ; v. 748 Springer Protocols (Springer-12345) Springer protocols (Series) |
| Subjects: |
Table of Contents:
- Screening for Ligands of C-Type Lectin-Like Receptors
- Yeast Surface Display of Lamprey Variable Lymphocyte Receptors (VLR)
- Identification of Scavenger Receptor Ligands
- Construction, Expression, and Purification of Chimeric Protein Reagents Based on Immunoglobulin Fc Regions
- Innate Immune Receptors for Nucleic Acids
- Analysis of Receptor-Ligand Interactions by Surface Plasmon Resonance
- Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2
- Determining FcεRI Diffusional Dynamics Via Single Quantum Dot Tracking
- Ratiometric Analysis of Subcellular Recruitment of Fc Receptors During Phagocytosis
- Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1
- Measuring Immune Receptor Mobility by Fluorescence Recovery after Photobleaching
- Probing the Plasma Membrane Structure of Immune Cells through the Analysis of Membrane Sheets by Electron Microscopy
- Rapamycin-Based Inducible Translocation Systems for Studying Phagocytosis
- Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling
- CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Antigens
- Use of Colloidal Silica-Beads for the Isolation of Cell-Surface Proteins for Mass Spectrometry-Based Proteomics
- Transfection-Based Genomic Readout for Identifying Rare Transcriptional Splice Variants
- Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System
- Characterizing Immune Receptors from New Genome Sequences
- Screening for ligands of c-type lectin-like receptors / Elwira Pyz and Gordon D. Brown
- Yeast surface display of lamprey variable lymphocyte receptors / Gang Xu, Satoshi Tasumi, and Zeev Pancer
- Identification of scavenger receptor ligands / Claudine Neyen, Annette Pluddemann, and Siamon Gordon
- Construction, expression, and purification of chimeric protein reagents based on immunoglobulin Fc regions / John P. Cannon, Marci O'Driscoll, and Gary W. Litman
- Innate immune receptors for nucleic acids / Andrea Stutz, Damien Bertheloot, and Eicke Latz
- Analysis of receptor-ligand interactions by surface plasmon resonance / Kimiko Kuroki and Katsumi Maenaka
- Cell-based reporter assay to analyze activation of Nod1 and Nod2 / Birte Zurek, Harald Bielig, and Thomas A. Kufer
- Determining FceRI diffusional dynamics via single quantum dot tracking / Diane S. Lidke [and others]
- Ratiometric analysis of subcellular recruitment of Fc receptors during phagocytosis / Patricia Mero and James W.D. Booth
- Assessment of the recycling of the membrane-bound chemokine, CX₃CL1 / Sajedabanu Patel, Ilya Mukovozov, and Lisa A. Robinson
- Measuring immune receptor mobility by fluorescence recovery after photobleaching / Kristen Silver and Rene E. Harrison
- Probing the plasma membrane structure of immune cells through the analysis of membrane sheets by electron microscopy / Bjorn F. Lillemeier and Mark M. Davis
- Rapamycin-based inducible translocation systems for studying phagocytosis / Michal Bohdanowicz and Gregory D. Fairn
- Micropatterned ligand arrays to study spatial regulation in Fc receptor signaling / Alexis J. Torres, David Holowka, and Barbara A. Baird
- CELLISA : reporter cell-based immunization and screening of hybridomas specific for cell surface antigens / Peter Chen, Aruz Mesci, and James R. Carlyle
- Use of colloidal silica-beads for the isolation of cell-surface proteins for mass spectrometry-based proteomics / Yunee Kim [and others]
- Transfection-based genomic readout for identifying rare transcriptional splice variants / Larry J. Dishaw [and others]
- Characterizing somatic hypermutation and gene conversion in the chicken DT40 cell system / Nagarama Kothapalli and Sebastian D. Fugmann
- Characterizing immune receptors from new genome sequences / Katherine M. Buckley and Jonathan P. Rast